Plasmid Preparation:Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids.
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000bp homologous arms at both ends was used to integrate the pheAfbr and aroFfbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000 bp homologous arms at both ends was used to integrate the pheA fbr and aroF fbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Negative Control:Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids.
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000bp homologous arms at both ends was used to integrate the pheAfbr and aroFfbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000 bp homologous arms at both ends was used to integrate the pheA fbr and aroF fbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Positive Control:Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids.
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000bp homologous arms at both ends was used to integrate the pheAfbr and aroFfbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000 bp homologous arms at both ends was used to integrate the pheA fbr and aroF fbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Construct:Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids.
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000bp homologous arms at both ends was used to integrate the pheAfbr and aroFfbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
Article Title: Repurposing conformational changes in ANL superfamily enzymes to rapidly generate biosensors for organic and amino acids
Article Snippet: .. To optimize the droplet microfluidic screening method, the pK18mobsacB plasmid with corresponding transcriptional translation elements and 1000 bp homologous arms at both ends was used to integrate the pheA fbr and aroF fbr genes with RBS “GGGGGGGGG” or “GAAAGGAG” into the genome of C. glutamicum ATCC 13032, and the phenylalanine low (Negative Control, NC) and high (Positive Control, PC) yield controls were constructed. .. Plasmids pK18mobsacB_NC and pK18mobsacB_PC were electroporated into C. glutamicum ATCC 13032.
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